Description
Laboratory Research Technician III
Med-Ophthalmology - Pennsylvania-Pittsburgh - (25007051)
A Research Tech III position is available in the Ait Ali/Sahel lab of the University of Pittsburgh, Ophthalmology Department.
Bachelor's degree in Sciences, Molecular biology preferred. 2 years of lab experience required.
Job Summary
Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.
Essential Functions
Research & Laboratory Expertise
• Bibliographic research and experimental design to test and validate scientific hypotheses
• Design, implementation, and optimization of experimental workflows; troubleshooting technical and biological parameters in multi-day experiments
• Method validation and design of comparative experimental conditions
Laboratory Management & Organization
• General laboratory organization, including protocol writing, inventory management, purchasing, and safety documentation
• Supervision and training of students
Cell Biology & Biochemistry
• Cell culture at small to large scale (tens to hundreds of millions of cells) for quantitative profiling experiments
• Cell harvest, lysis, protein extraction, solubilization, fractionation, and protein biochemistry
• Cell line maintenance, passaging, cryostorage, harvesting, aliquot preparation, and mycoplasma testing
Molecular Biology & Microbiology
• Plasmid preparation, competent cells, agar plates, and plaque assays
• Bacteriophage detection, preparation of bacterial growth media, buffers, and aliquots
• Nucleic acid extraction, purification, and quantification, PCR, cDNA synthesis, and RT-PCR
Protein & Biochemical Assays
• Western blotting, BCA protein assay, calcium measurement assays, Protein extraction
Animal Models
• Mouse (Mus musculus) handling
• Retina extraction and embedding in OCT compound
• Ear punching and tail sampling, organ dissection, and cryostorage
• Injections (intracardiac, subcutaneous, intraperitoneal, retro-orbital), anesthesia, euthanasia
• Genotyping by PCR
Software & Databases
• Excel, ImageJ, Prism, SnapGene
• NCBI, Ensembl, Primer3
• Photoshop, Adobe Illustrator, EndNote
Physical Effort
Be able to work at lab bench for hours at a time, ability to lift up to 25lbs
Assignment Category: Full-time regular
Job Classification: Staff.Laboratory Research Technician III
Job Family: Research
Job Sub Family: Laboratory
Campus: Pittsburgh
Minimum Education Level Required: Bachelor's Degree
Minimum Years of Experience Required: 2
Will this position accept substitution in lieu of education or experience: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement.
Work Schedule: M- F 8:30am to 5:00pm
Work Arrangement: On-Campus: Teams that work on campus, in an office, or in a lab.
Hiring Range: TBD Based Upon Qualifications
Relocation_Offered: No
Visa Sponsorship Provided: No
Background Check: For position finalists, employment with the University will require successful completion of a background check
Child Protection Clearances: Not Applicable
Required Documents: Resume
Optional Documents: Cover Letter
PI280916384